Chongqing High Performance Liquid Chromatography SystemTechnical Parameter

1. Overall requirements: The entire system consists of a high-pressure infusion pump, an automatic sampler, a column temperature chamber, and a UV detector.
2. High pressure infusion pump: Four element gradient pump, series double piston reciprocating pump, low-pressure gradient mixing. New floating piston design; Online piston cleaning design. The pressure pulsation of the pump is extremely small and does not require a pulsation damper.
a) Number of solvent channels: 4
b) Flow rate range: 0-10ml/min, increasing by 0.001ml/min
c) Pressure range: 0.1-62MPa
d) Flow rate accuracy: ± 0.1% (1.0ml/min)
e) Flow velocity accuracy:<0.05% RSD
f) Gradient accuracy: ± 0.5% (2ml/min)
g) Gradient accuracy:<0.2% SD (2ml/min)
h) Gradient delay volume: 690 μ L, does not vary with backpressure changes
i) Gradient composition ratio: 0-100%
j) Leakage sensor: Built in leakage sensor
3. Online vacuum degassing machine
a) Built in 4-channel vacuum degassing machine
b) Maximum flow rate: 14ml/min
c) Volume per channel: 670 μ L
d) PH tolerance range: 1-14
4. Automatic sampler: The injection needle is integrated into the high-pressure flow path; After injection, the injection needle is placed in the flow path, and during analysis, there is always a mobile phase flowing through the needle, resulting in minimal sample residue; Standard configuration with independent injection needle external cleaning function, minimal cross contamination.

a) Injection accuracy: ± 0.5%
b) Injection accuracy:<0.25% RSD
c) Cross contamination:<0.005%
d) Injection volume range: 0.01-100 μ L, up to 2500 ul with large syringe and injection ring (optional)
e) Multiple sample tray options: 120 x 1.8 ml or 2ml sample bottles; 3 x 96 conventional and deep hole plates;
3 x 384 deep hole plate
f) Online dilution and online derivative functions

5. Column temperature box
a) Temperature range: 5-110 ℃, 18 ℃ at room temperature
b) Temperature accuracy: ± 0.5 ℃
c) Temperature accuracy: ± 0.1 ℃
d) Column temperature chamber capacity: It can accommodate up to 6 chromatography columns with a length of 300 mm
e) Pre column heating volume: 7 μ L
f) Column switching: There are 2 reserved positions for column switching valves, which can be configured with corresponding six way or ten way valves as needed to complete advanced applications such as series analysis, parallel analysis, online sample pretreatment, or method switching
6. UV detector
a) Wavelength range: 190-800nm
b) Noise: ＜ ±0.25x10-5AU（254nm）
c) Drift:< 1x10-4AU/h（254nm）
d) Spectral bandwidth: 6nm
e) Wavelength accuracy: ± 1nm
f) Wavelength repeatability: ± 0.1nm
g) Data collection frequency: 100Hz
h) Light source: deuterium lamp, tungsten lamp, dual lamp position, deuterium lamp tungsten lamp switching software completed, no need for manual lamp replacement.
7. Data processing software
a) Operating System: Compatible with Windows XP windows Vista、Windows 7
b) Chromatographic Control Analysis Database: Through high-performance USB and computer digital signal transmission, analysis methods and sequences can be programmed, compatible with multiple database platforms such as Microsoft Access, Oracle, and Microsoft SQL Server
c) Can automatically perform fast data collection and post-processing
d) Can provide timely analysis of condition parameters and analysis results, online monitoring and collection of pump pressure change data
e) Equipped with instrument related data and operation status traceability function, convenient for troubleshooting
f) It can be upgraded to be compatible with third-party instruments and can be upgraded to network software to operate third-party instrument companies including gas chromatography, ion chromatography, etc
g) Experimental results can be output in formats such as PDF and EXCEL. The relevant operations for editing experimental data are in EXCEL format, with flexible calculations and easy modification

Chongqing High Performance Liquid Chromatography System